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Part:BBa_M36580:Experience

Designed by: Elisa Vidales   Group: Stanford BIOE44 - S11   (2015-10-22)
Revision as of 21:10, 7 December 2015 by Evidales (Talk | contribs)

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Please enter how you used this part and how it worked out.


Stanford Location

Glycerol Stocks

Barcode #: 0133010047

Plasmid Name: qsc102

Antibiotic resistance: kanamycin

DNA 2.0 gene #: 232006

Organism expressed in: E. coli

Sensor


Barcode #: 0133010047

Plasmid Names (double transformed): LasR + qsc102

Antibiotic resistance: ampicillin + kanamycin

DNA 2.0 gene #: 232005; 232006

Organism expressed in: E. coli

Double Plasmid Actuator/Sensor

Applications of BBa_M36580

Used in conjunction with BBa_M36575 in BIOE44 at Stanford in attempts to create a P. aeruginosa biosensor.

User Reviews

UNIQf20197578e12c340-partinfo-00000000-QINU UNIQf20197578e12c340-partinfo-00000001-QINU

We have little data on the functionality of this construct since it was used in conjunction with BBa_M36575, which proved inviable. However, what we do know is that a low amount of fluorescence was observed in all cells with the qsc102 plasmid, despite a lack of LasR presence (Graph 1). The promoter is likely leaky, and transcribed the GFP gene following it even without presence of the LasR-PAI1 complex.

Graph 1.png

Graph 1. Normalized fluorescence levels for GFP expression in the double-transformed E. coli