Composite

Part:BBa_M36580:Design

Designed by: Elisa Vidales   Group: Stanford BIOE44 - S11   (2015-10-22)
Revision as of 23:35, 23 October 2015 by Evidales (Talk | contribs) (→‎Design Notes)


LasR/PAI complex sensor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Added preceding spacing to qsc102 promoter palindromic binding sequence (included in qsc102 part), as well as spacing flanking the RBS in order to optimize binding. The added spacing sequences are detailed below.

Sequence before RBS: CAGGATTACA Sequence after RBS: TAACCTATCG

Source

qsc102 promoter: Boxed region from Figure 1 of http://www.ncbi.nlm.nih.gov/pmc/articles/PMC95443/ Whiteley spacing: Region following boxed region to bolded A of http://www.ncbi.nlm.nih.gov/pmc/articles/PMC95443/ BBa_0030 strong RBS: partsregistry.org GFP coding sequence: BIOE44 pComet DNA sequence

References