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Protein_Domain
Part:BBa_M36142:Experience
Designed by: Tatyanna Dadabbo & Richard Hojel & Soraya Karimi Group: Stanford BIOE44 - S11 (2015-10-22)
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Applications of BBa_M36142
This DNA sequence for the Mature EP-B2 was the correct sequence for the protein. When conducting a fluorescence assay for the protein in lysed cells, the results proved that the correct protein was being cleaved, therefore the mature EP-B2 protein was present. The assay was successful as the mature EP-B2 protein was necessary to cleave the fluorescent part of the quencher.
When designing our constructs, we chose to make two constructs, one with the mature EP-B2 sequence and one with the mature EP-B2 sequence. We were testing whether the inhibitor sequence in the Pro EP-B2 was necessary for the protein to be made. Given our successful results for the Mature EP-B2 (with no inhibitor sequence) from the lysed cells fluorescence assay, we concluded that the inhibitor sequence is not necessary for the protein to be made.
User Reviews
UNIQ1f561fe25a02d89e-partinfo-00000000-QINU UNIQ1f561fe25a02d89e-partinfo-00000001-QINU