Coding

Part:BBa_K1689018:Design

Designed by: Wei weijia   Group: iGEM15_Peking   (2015-09-16)
Revision as of 15:48, 27 September 2015 by Dawnec (Talk | contribs) (→‎Design Notes)

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dCas9-F3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1150
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4
    Illegal BamHI site found at 3429
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The coding sequence of this fusion protein is very long. If you want to amplify only part of it (eg. dCas9 only), you’d better dissect the part you need into several segments, amplify them separately, and rejoin them using Gibson Assembly.

Source

E.coli

References