Coding

Part:BBa_K1689020:Design

Designed by: Wei weijia   Group: iGEM15_Peking   (2015-09-16)
Revision as of 15:19, 27 September 2015 by Dawnec (Talk | contribs) (→‎Source)


dCas9-delta omega


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1150
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4
    Illegal BamHI site found at 3429
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The coding sequence of this fusion protein is too long, you'd better dissect it into several segments and amplify it separately.

Source

E.coli

References