Generator

Part:BBa_K1859028:Experience

Designed by: Yusuke Banno   Group: iGEM15_Gifu   (2015-09-10)
Revision as of 05:15, 25 September 2015 by Kozakai (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.


Applications of BBa_K1859028



We inserted this generator into E. coli K-12 JM109.
We cultivated this bacteria and performed SDS-PAGE without boiling.
Lane D shows this sample.


left: SDS-PAGE before dye / center: SDS-PAGE after dye / right: overlay

There was no fluorescence. We found that this generator doesn’t make functional long chain protein.

User Reviews

UNIQ7bdb3f558ef2b3ab-partinfo-00000001-QINU UNIQ7bdb3f558ef2b3ab-partinfo-00000002-QINU