Composite
cI-Cro+
Part:BBa_K1846005:Design
Designed by: Luba Prout, Vitor Pinheiro Group: iGEM15_Birkbeck (2015-09-17)
cI-Cro bacteriophage lambda lytic cycle regulatory circuit
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This cI-Cro circuit was synthesised removing the illegal restriction sites to make the sequence BioBrick-compatible. The sequence was cloned into a pSB1C3 and the success of the cloning procedure was confirmed by restriction with EcoRI and SpeI restriction enzymes and followed by agarose gel electrophoresis (Figure 1). The construct was also confirmed by sequencing. This BioBrick was registered as part BBa_K1846005.
Source
DNA synthesis