Composite

Part:BBa_K1649002

Designed by: Yang Yang   Group: iGEM15_NJAU_China   (2015-09-07)
Revision as of 18:15, 20 September 2015 by Yangshulantian (Talk | contribs)

Red light sensor with TetR not gate and GFP reporter

As the transcription from the red light sensor is repressed by the function of red light and activated by dark, we use a not gate, repressor TetR and tetr operator to ensure the system is activated by red light.GFP can be used to test whether the circuit works.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 5318
    Illegal XhoI site found at 382
    Illegal XhoI site found at 2646
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4871
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 7067


[edit]
Categories
Parameters
None