Composite

Part:BBa_K1598005:Design

Designed by: Marta Napiorkowska   Group: iGEM15_UCL   (2015-09-17)
Revision as of 17:00, 20 September 2015 by Lpvc (Talk | contribs) (→‎Design Notes)

pYear-RBS-TPH1-6xHis-Terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part consists of the pYeaR promoter: <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K216005">BBa_K216005</a>, an RBS, the TPH1 expression gene: Part:BBa_K216005 and a terminator.

- human TPH1 gene was codon-optimized for E.coli and provided with C-terminal 6xHis tag - sequence was synthesized in the form of gBlock and cloned into pSB1C3

Source

DNA synthesis

References