RNA

Part:BBa_K1777008

Designed by: Xilin Jiang   Group: iGEM15_Fudan   (2015-09-15)
Revision as of 12:55, 20 September 2015 by YANG1126 (Talk | contribs) (→‎Fuction)

RNA accelerating cyclization

This RNA can bind to the flanking region of exon4 of hsACTB in HEK293T cells and facilitate the back-splicing mechanism. This part can be used to cyclize certain RNA exon.


Fuction

We design this RNA to promote the cyclization of hsACTB in HEK293T cells.
HsACTB.JPG

Results

Forty-eight hours after transfection with pZW1-sno-ACTB into HEK 293T cells, we use the specific circular exon-4 primers to test the circular RNA.
Unexpectedly, we amplify other circular RNAs using specific primers.(BLAST on UCSC-In Silico, proved to be specific）
AcRNA-ACTB.jpg
Both vector without insert pZW1-sno and plasmid with acRNA pZW1-sno-ACTB appear non-specific electrophoretic band. After sequencing, we found 200bp: circular RNA consists of exon4-exon3-exon4, 500bp: circular RNA onsists of exon4-exon5-exon6-exon2-exon3-exon4 while 700bp: circular RNA consists of exon4-exon5-exon4-exon4. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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