Protein_Domain
Part:BBa_K1486003
Designed by: iGem EPFL 2014 Group: iGEM14_EPF_Lausanne (2014-08-12)
Revision as of 08:14, 20 September 2015 by Xuhangli (Talk | contribs) (→Applications of BBa_K1486003)
Flexible linker 2x (GGGS)
Flexible linker used to fuse two proteins.
Applications of BBa_K1486003
Group: iGEM15_Fudan
Author: Xuhang Li
Summary:We developed a new part(BBa_K1777005) based on this parts.When we planned to use this part to link our fusion component,we found it hard to design a proper primer to amplify it because of mutiple primer binding site as a result of tandem GGGS.Therefore, we first improved it by change the first GGGS to a natural linker obtained from linker database.In addition,the codon is optimized for mammalian, and we insert a NLS to promote locating in cell nucleus. Such an improved GS linker could be easily assembled to your protein via overlap PCR and offer the property of nuclear localization.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
//direction/forward
//proteindomain/linker
//proteindomain/linker
chassis | E coli |
device_type | linker |
direction | forward |