Terminator

Part:BBa_K1725081

Designed by: Mhairi Davidson   Group: iGEM15_Glasgow   (2015-08-11)
Revision as of 22:48, 19 September 2015 by Scolloms (Talk | contribs)

T1 terminator from E. coli rrnB

  • T1 terminator from E. coli rrnB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

This terminator was tested using pSB1A10, a terminator testing plasmid.

Glasgow_2015_pSB1A10.png

Our results show that insertion of K1725081, reduces RFP expression compared to no terminator, and gives approximately equal termination to terminators B0010 and B0015.

Glasgow_2015_Terminator_Scan_and_Graph.png

[edit]
Categories
Parameters
None