Regulatory
p1997

Part:BBa_K1820000

Designed by: Thomas Overbeck   Group: iGEM15_Utah_State   (2015-09-16)
Revision as of 16:12, 26 September 2015 by Szeyvion (Talk | contribs) (→‎Usage and Biology)

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P335-Φ31 inducible middle promoter

A phage-inducible promoter natively located in the middle of the genome of the P335 species lactococcal phage Φ31, containing two tandem transcription sites.

Usage and Biology

Transcription from this promoter is induced after the phage genome is inserted into the bacterial cell, resulting in downstream expression in the initial stages of infection. It has been previously demonstrated that expression is successfully induced from this promoter and only occurs during phage infection.

This construct has yet to be cloned and expressed in L. lactis, and validated for functionality.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 42


References

Djordjevic GM, Klaenhammer TR (1997) Bacteriophage-triggered defense systems: phage adaptation and design improvements. Appl Environ Microbiol 63:4370–4376.

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