Composite
RBS-GFP_Si

Part:BBa_K1602025

Designed by: Jasmin Weber, Michelle Zoeller, Marcus Geissler, Stefan Bohn, Simon Linder, Sebastian Barthel   Group: iGEM15_TU_Darmstadt   (2015-09-16)
Revision as of 02:10, 19 September 2015 by Jweber (Talk | contribs)

HisTag-GFP_Si4-Tag fusion protein with RBS BBa_B0034

This part is encodes for the green fluorescent protein (GFP). It also contains a strong Ribosome Binding Site. Also the sequence of a Histag is fused to the gene sequence, improving the purification yield. Fusing the Si4-tag(BBa_K1028000) to the 3' end of the sequence enables the immobilization of the multi-complex enzyme to a silica surface.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 680


[edit]
Categories
Parameters
None