Coding

Part:BBa_K1796011

Designed by: Nannan Xie   Group: iGEM15_SCU_China   (2015-09-17)
Revision as of 02:02, 19 September 2015 by Nianhui (Talk | contribs) (→‎Parameter of Protein)

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nifN promoted from Paenibacillus sp. WLY78

Function: scaffold gene of nitrogen fixation system

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 319
    Illegal PstI site found at 890
    Illegal PstI site found at 961
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 319
    Illegal PstI site found at 890
    Illegal PstI site found at 961
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 319
    Illegal PstI site found at 890
    Illegal PstI site found at 961
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 319
    Illegal PstI site found at 890
    Illegal PstI site found at 961
    Illegal NgoMIV site found at 648
    Illegal AgeI site found at 25
    Illegal AgeI site found at 147
    Illegal AgeI site found at 766
    Illegal AgeI site found at 987
  • 1000
    COMPATIBLE WITH RFC[1000]


Parameter of Protein

Number of amino acids: 467

Molecular weight: 50684.0

Theoretical pI: 7.21

Amino acid composition: 

Ala (A) 45 9.6%

Arg (R) 30 6.4%

Asn (N) 10 2.1%

Asp (D) 22 4.7%

Cys (C) 8 1.7%

Gln (Q) 14 3.0%

Glu (E) 25 5.4%

Gly (G) 41 8.8%

His (H) 15 3.2%

Ile (I) 21 4.5%

Leu (L) 56 12.0%

Lys (K) 17 3.6%

Met (M) 11 2.4%

Phe (F) 15 3.2%

Pro (P) 23 4.9%

Ser (S) 44 9.4%

Thr (T) 23 4.9%

Trp (W) 8 1.7%

Tyr (Y) 9 1.9%

Val (V) 30 6.4%

Pyl (O) 0 0.0%

Sec (U) 0 0.0%

(B) 0 0.0%

(Z) 0 0.0%

(X) 0 0.0%

Total number of negatively charged residues (Asp + Glu): 47

Total number of positively charged residues (Arg + Lys): 47

Atomic composition:Carbon C 2246

Hydrogen H 3570

Nitrogen N 636

Oxygen O 662

Sulfur S 19

Formula: C2246H3570N636O662S19Total number of atoms: 7133

Extinction coefficients:Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water.

Ext. coefficient 57910

Abs 0.1% (=1 g/l) 1.143, assuming all pairs of Cys residues form cystines

Ext. coefficient 57410

Abs 0.1% (=1 g/l) 1.133, assuming all Cys residues are reduced

Estimated half-life:The N-terminal of the sequence considered is T (Thr).

The estimated half-life is: 7.2 hours (mammalian reticulocytes, in vitro).

>20 hours (yeast, in vivo).

>10 hours (Escherichia coli, in vivo).

Instability index:The instability index (II) is computed to be 41.38

This classifies the protein as unstable.

Aliphatic index: 92.57

Grand average of hydropathicity (GRAVY): -0.051

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