Generator

Part:BBa_K1820020

Designed by: Sara Gertsch   Group: iGEM15_Utah_State   (2015-09-16)
Revision as of 01:37, 19 September 2015 by Saragertsch (Talk | contribs)

P335-Φ31 inducible middle promoter + RBS+ sfGFP + term

A phage-inducible promoter natively located in the middle of the genome of the P335 species lactococcal phage Φ31 with the B0034 ribosomal binding site (RBS) (BBa_K1820001), a super-folded green fluorescent protein (sfGFP) from the 2014 Groningen team that was codon optimized for Bacillus subtilis (BBa_K1365020), and a transcriptional terminator.

Usage and Biology

Transcription from this promoter is induced after phage infection of Lactococcus lactis, resulting in expression of sfGFP (previously shown to have a high level of fluorescent activity in L. lactis). Allowing for fluorescent detection of Φ31 phage infection in L. lactis.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 42
    Illegal SapI.rc site found at 284


[edit]
Categories
Parameters
None