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Generator
Part:BBa_K1820020
Designed by: Sara Gertsch Group: iGEM15_Utah_State (2015-09-16)
Revision as of 01:18, 19 September 2015 by Saragertsch (Talk | contribs)
P335-Φ31 inducible middle promoter + RBS+ sfGFP + term
A phage-inducible promoter natively located in the middle of the genome of the P335 species lactococcal phage Φ31 with the native ribosomal binding site (RBS) (BBa_K1820001), a super-folded green fluorescent protein (sfGFP) that was codon optimized for Bacillus subtilis (BBa_K1820004), and a transcriptional terminator.
Usage and Biology
Transcription from this promoter is induced after phage infection of Lactococcus lactis, resulting in expression of sfGFP (previously shown to have a high level of fluorescent activity in L. lactis). Allowing for fluorescent detection of Φ31 phage infection in L. lactis.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 42
Illegal SapI.rc site found at 284
[edit]
Categories
Parameters
None |