Generator

Part:BBa_K1807007

Designed by: Ivan Gyulev   Group: iGEM15_York   (2015-09-17)
Revision as of 00:52, 19 September 2015 by Isgyulev (Talk | contribs)

Synthetic Phosphate-specific Transporter Operon

This part enables the inducible expression of a synthetically designed (codon optimized and re-factored) Accumulibacter Phosphate-specific transporter (the amino acid sequence has been derived from the Accumulibacter phosphatis SK-1 strain).


Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 2133
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 529
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 655
    Illegal NgoMIV site found at 716
    Illegal NgoMIV site found at 1732
    Illegal NgoMIV site found at 2305
    Illegal AgeI site found at 164
    Illegal AgeI site found at 2762
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1417
    Illegal BsaI site found at 2383
    Illegal SapI site found at 1223
    Illegal SapI.rc site found at 3169


[edit]
Categories
Parameters
None