Coding

Part:BBa_K1583001

Designed by: Stefan Robert Marsden   Group: iGEM15_TU_Delft   (2015-09-04)
Revision as of 00:18, 19 September 2015 by Maxvthof (Talk | contribs)

CsgC

CsgC prevents CsgA proteins from self-assembling inside the cell. CsgA is a protein monomer which can aggregate to form amyloid nanowires in natural biofilms of E.coli (Barnhart & Chapman 2010).

csgC is used in the biobricks:

The first three parts contain CsgC with a low constitutive promoter (BBa_J23110), a medium constitutive promoter (BBa_J23118) and a high consitutive promoter (BBa_J23100).

The other three parts contain both CsgC and CsgB. These induced are by a low constitutive promoter (BBa_J23110), a medium constitutive promoter (BBa_J23118) and a high consitutive promoter (BBa_J23100).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

Barnhart, M. M., & Chapman, M. R. (2010). Curli Biogenesis and Function, 4945.

[edit]
Categories
Parameters
None