Part:BBa_K1583001

CsgC

CsgC prevents CsgA proteins from self-assembling inside the cell. CsgA is a protein monomer which can aggregate to form amyloid nanowires in natural biofilms of E.coli (Barnhart & Chapman 2010).

It is used in the biobricks:

• <a href="https://parts.igem.org/Part:BBa_K1583200">BBa_K1583200</a>
• <a href="https://parts.igem.org/Part:BBa_K1583201">BBa_K1583201</a>
• <a href="https://parts.igem.org/Part:BBa_K1583202">BBa_K1583202</a>
• <a href="https://parts.igem.org/Part:BBa_K1583203">BBa_K1583203</a>
• <a href="https://parts.igem.org/Part:BBa_K1583204">BBa_K1583204</a>
• <a href="https://parts.igem.org/Part:BBa_K1583205">BBa_K1583205</a>

The first three parts contain CsgC with a low constitutive promoter (<a href="https://parts.igem.org/Part:BBa_J23110">BBa_J23110</a>), a medium constitutive promoter (<a href="https://parts.igem.org/Part:BBa_J23118">BBa_J23118</a>) and a high consitutive promoter (<a href="https://parts.igem.org/Part:BBa_J23100">BBa_J23100</a>).

The other three parts contain both CsgC and CsgB. These induced are by a low constitutive promoter (<a href="https://parts.igem.org/Part:BBa_J23110">BBa_J23110</a>), a medium constitutive promoter (<a href="https://parts.igem.org/Part:BBa_J23118">BBa_J23118</a>) and a high consitutive promoter (<a href="https://parts.igem.org/Part:BBa_J23100">BBa_J23100</a>).

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References

Barnhart, M. M., & Chapman, M. R. (2010). Curli Biogenesis and Function, 4945.