Device

Part:BBa_K1679030:Design

Designed by: Xihan Zhang   Group: iGEM15_OUC-China   (2015-09-18)
Revision as of 22:46, 18 September 2015 by Yefeng (Talk | contribs) (→‎Design Notes)

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J23119 + I13504 + J23119 + K115002 + E1010


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 933
    Illegal NheI site found at 956
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1582
    Illegal AgeI site found at 1694
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 705


Design Notes

The same promoter can control the quantity of transcription, so that the ratio of GFP and mCherry is related to RNA thermometer.

Source

Assembled parts from Registry through 3A assembly.

References