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Part:BBa_K1723008:Experience

Designed by: Emilie Cuillery   Group: iGEM15_EPF_Lausanne   (2015-09-15)
Revision as of 20:34, 18 September 2015 by E.Cuillery (Talk | contribs)

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Applications of BBa_K1723008




EPF_Lausanne_transistor.png

This picture represent the system we want to build using this promoter: a transistor like element in cell. The complex dCas9-sgRNA will bind on the promoter and activate or inhibit it.




Effects of dCas9-ω BBa_K1723000 on BBa_K1723005 promoter

EPF_Lausanne_RESULTS_EXPERIENCE2.png

promoter strength was assessed using GFPmut2 reporter.


This chart present the effects of dCas9-ω (BBa_K1723000) on the PAM rich URS J23117Alt promoter (BBa_K1723005) when provided following sgRNAs:

[A_alt] sgRNA X4 (BBa_K1723007), activating
[R2_alt] sgRNA X0 (BBa_K1723006), inhibiting
[R1_alt] sgRNA X35 (BBa_K1723008), inhibiting

In comparison with:

[B_alt] apo-dCas9-ω (=no sgRNA)
[Af_alt] apo-dCas9-ω (=no sgRNA), no reporter


This chart show the response of the promoter PAM rich URS J23117Alt (BBa_K1723001) in the presence of different complexes sgRNA-dCas9-ω provided by our part collection. Specifically, we see that in presence of the complex BBa_K1723008-dCas9-ω, the promoter PAM rich URS J23117 (BBa_K1723001) is not activated. Visit our website for more informations: http://2015.igem.org/Team:EPF_Lausanne




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