Coding
LuxI-His

Part:BBa_K1709000:Design

Designed by: Astrid Deryckere   Group: iGEM15_KU_Leuven   (2015-09-08)
Revision as of 17:25, 18 September 2015 by AstridD (Talk | contribs) (→‎Design Notes)

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LuxI-His


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Biobrick BBa_C0061 still contained a Barcode sequence which was deleted. For our own use, 5 bp were exchanged to create unique restriction sites and to optimize DNA folding.

Source

Sequence is originating from V. fischeri. Here, the sequence from BBa_C0061 was used and optimized.

References