Part:BBa_K1714001

This part will be finally introduced upstream of BBa_K1714004 (PBAD-B0034-BLA-dT on 1C3) and expressed as AMPs in Co-expression system. In this page, I described the result of the activity assay of ABF-2.

In this page, I described the result of the activity assay of ABF-2.

We transformed it into E. coli (in this case, we used DH5α) and compared the growth curve between samples induced or not.

Activity test for ABF-2 and thanatin

→Comparing growth curve between the samples one is induced by IPTG and the other is not induced.

1. Diluting the bacterial culture which is enough pre-cultured by 0.1 OD₆₀₀ (Optical Density in λ=600nm) in 2 mL LBC.(Lysogeny Broth liquid culture including chloramphenicol)
2. Adding 2 µL of IPTG into one and DW into the other.
3. Measuring OD₆₀₀ of these samples once in an hour.

As a result, we got the graph of growth curve of DH5α and shown on below as Chart1.

<img src="" class="figure">

Fig. 8. The growth curve of E. coli including <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1714001" target="blank">BBa_K1714001</a> with IPTG or not

As you can see, we can’t say that ABF-2 in <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1714001" target="blank">BBa_K1714001</a> has correct antimicrobial activity.