Part:BBa_K1819002

TAT signal

Twin-arginine translocation signal (TAT signal) can be used to protein secretion to the extracellular environment of Escherichia coli. We designed TAT signal based on Yikmis et al¹ paper that describes a TAT signal from HyaA (hydrogenase 1 small subunit) been used to export Lcp .

SacI restriction site was added at the 3’ flanking region of TAT signal to link it at the first amino acid residue of the protein to be exported.

Schematic representation of BBa_K1819002 insert.

Please note this part was submitted in pSB1C3, the Registry's standard shipping backbone, according to submission requirements. However, pSB1C3 contains a SacI site and if you want to use this part we recommend to move the part into another plasmid backbone what can be easily done.

References

1. Yikmis, M. et al. Secretion and transcriptional regulation of the latex-clearing protein, lcp, by the rubber-degrading bacterium Streptomyces sp strain K30. Appl. Environ. Microbiol. 74, 5373-5382, doi:10.1128/aem.01001-08 (2008).

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]