Coding

Part:BBa_K1583104

Designed by: Stefan Robert Marsden   Group: iGEM15_TU_Delft   (2015-09-04)
Revision as of 15:43, 18 September 2015 by SMarsden (Talk | contribs)

pRha + Mfp5_CsgA_His fusion protein

CsgA is a protein monomer which can aggregate to form amyloid nanowires in natural biofilms taken from E.coli K-12 MG1655. Inspired by mussels, the Mfp5 (mussel foot protein) has high adhesive properties towards wet polar surfaces. By creating a fusion protein, the adhesive properties of the mussel foot protein is combined with the formation of nanowires.

The design was based on the paper "Strong underwater adhesives made by self-assembling multi-protein nanofibres".[1]

1. C.Zhong, T.Gurry, A.Cheng, J.Downey, Z.Deng, C. Stultz, T.Lu, Nature Nanotechnology, 2014, 9, 858-866.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 245
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//awards/part_collection/2015
Parameters
None