Coding

Part:BBa_K1593998

Designed by: Yuting Liu   Group: iGEM15_USTC   (2015-09-03)
Revision as of 15:29, 18 September 2015 by Yukae (Talk | contribs)

overexpress cheZ

this part is uesd for coding cheZ,a chemotactic protein.


Stracture

PDB code:1KMI

4901.jpg


Growth characteristics

We detected OD600(nm) to identify the effect of cheZ expression on E. coli through time.

4902.jpg

The exogenous protein expression affects the growth of bacteria, which was indicated by slight slower grow-up in E. coli containing cheZ plasmid. In total, cheZ doesn't have obvious negative impact on bacteria grown.


SDS-PAGE

After induced by IPTG in 1mM about 4 h, cheZ was found in SDS-PAGE gel, compared to the wild type (Top10) and bacteria without IPTG induction. Through this result, we concluded that cheZ has been successfully expressed with the IPTG induction.

4903.jpg



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 373
    Illegal NgoMIV site found at 508
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None