Composite

Part:BBa_K1157006:Experience

Designed by: Lin Ang Chieh   Group: iGEM13_NTU-Taida   (2013-09-10)
Revision as of 14:59, 18 September 2015 by Karkugv14 (Talk | contribs) (→‎User Reviews)

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1157006

We used flow cytometry to test the individual fluorescence expression of our biobrick from 0 to 4 hours and in different concentration(10-3, 10-5,10-7). Flow mCherry.jpg

User Reviews

UNIQ64bb5f938a15b164-partinfo-00000000-QINU

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iGEM_Stockholm_2015

Method: Promoter, RBS, RhlI, and double terminator BioBricks were transformed in TOP10 E. coli using the transformation protocol and were supposed to be assembled using the 3A assembly protocol.

Results: During the initial lab weeks there were some trouble with transformation, later addressed to not using KCM buffer. The BBa_K082035 was ordered from IDT to speed up the process after repeated failed transformations and transformation troubleshooting were done in parallel.

Conclusion: BBa_K082035 was ordered instead of cloned.

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