Coding

Part:BBa_K1593210

Designed by: Juntao Yu   Group: iGEM15_USTC   (2015-08-17)
Revision as of 14:43, 18 September 2015 by Yukae (Talk | contribs)

T7-OprF

Using T7 strong promoter to express OprF, derived from Pseudomonus areuginosa, would strongly improve the bacterial permeability.


T7

Information of T7:see BBa_I712074


OprF

Informtion of OprF:see BBa_K1593209

Growth Characterization

This is in order to demonstrate that the overexpression of porin won't severely affect the growth of bacteria, at least it won't significantly inhibit bacteria growth and become as kill switch. 4900.jpg

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 654
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None