Translational_Unit
yeast uS12

Part:BBa_K1729001

Designed by: Dani Bauhan and Richard Anthony   Group: iGEM15_RHIT   (2015-09-14)
Revision as of 13:38, 18 September 2015 by RicRHIT (Talk | contribs)

Yeast mitochondrial ribosomal protein MRPS12

This translational unit includes the Kozak and codon-optimized sequence for the Saccharomyces cerevisiae [http://www.yeastgenome.org/locus/S000005319/overview MRPS12] gene, which encodes a ribosomal protein of the small subunit of the mitochondrial ribosome. The coding sequence begins with a mitochondrial localization signal (MLS), which is presumably removed in the mitochondrion. The resultant protein is a homologue of the highly conserved uS12 ribosomal protein (Ban et al.), which is essential for ribosome function.


The part sequence corresponds to nucleotides 694840-694361 on chromosome XIV of the S. cerevisiae S288C reference genome.

MRPS12 Seq.jpg

Screen capture from SnapGene®


References

Ban, Nenad et al. “A New System for Naming Ribosomal Proteins.” Current opinion in structural biology 24 (2014): 165–169.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 442
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None