Composite

Part:BBa_K1736000:Design

Designed by: Nick Coleman   Group: iGEM15_Sydney_Australia   (2015-09-17)
Revision as of 12:47, 18 September 2015 by SydneyUni Australia (Talk | contribs) (→‎Design Notes)


Harmonised B. subtilis Falvin Binding Fluorescent Protein (BsFP)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 109
    Illegal BamHI site found at 229
    Illegal XhoI site found at 425
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The BsFP protein was harmonised by the method outlined in Spencer et al. 1. To download and obtain instructions on how to use the algorithm to harmonise your sequence, refer to the Sydney 2015 [http://2015.igem.org/Team:Sydney_Australia/Modeling modelling page]. Note that the part also contains an B.subtilis RBS.


1 Spencer P et al., 2012, “Silent Substitutions Predictably Alter Translation Elongation Rates and Protein Folding Efficiencies”, Vol 422, pp. 328-335.

Source

Bacillus Subtilits

References