Coding

Part:BBa_K1603004

Designed by: John Hellgren   Group: iGEM15_Chalmers-Gothenburg   (2015-09-18)
Revision as of 14:29, 18 September 2015 by Jannie (Talk | contribs)

SSB - optimized for yeast

Single strand binding protein from Deinococcus Radiodurans. Codon optimized for Saccharomyces cerevisiae and fused with a nuclear localization signal (NLS) for in vivo DNA repair. Also fused with a His-tag, allowing nickel based purification prior in vitro DNA repair


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 248
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 248
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 111
    Illegal XhoI site found at 875
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 248
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 248
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 482


[edit]
Categories
Parameters
None