Coding

Part:BBa_K1640023

Designed by: Meghan Cook   Group: iGEM15_Macquarie_Australia   (2015-09-18)
Revision as of 23:54, 18 September 2015 by Zac B (Talk | contribs)

psbO


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 754
  • 1000
    COMPATIBLE WITH RFC[1000]

Overview

This biobrick contains one gene from the photosystem II complex of Chlamydomonas reinhardtii - psbO, with RBS.

This part was designed in conjunction with BBa_K1640022 and BBa_K1640012 and together they form operon 5 in our set of photosystem II operons:

PSII diagram

Biology & Literature

PsbO encodes psbO, a 33 kDa protein which acts to stabilise the cluster of four Mn2+ which forms the catalytic centre of the oxygen evolving complex (OEC) (Murata & Miyao, 1985). PsbO consists of an eight strand β-barrel, with a large loop between strands five and 6, which connects the OEC to the luminal surface (Ferreira, Iverson, Maghlaoui, Barber, & Iwata, 2004). Deletion studies of psbO in Synechocystis sp. have shown little effect to photoautotrophic growth and oxygen evolution, however a markedly higher susceptibility to photoinhibition (Mayes, Cook, Self, Zhang, & Barber, 1991). This suggests PsbO is not essential to PSII assembly or water-splitting, however provides protection of PSII from light-induced damage.

Protein information

mass: 27.96kDa

sequence:
MAQKVGQAAAAAALATAMVAGSANALTFDEIQGLTYLQVKGSGIANTCPVLESGTTNLKELKAGSYKLENFC IEPTSFTVKEESQFKGGETEFVKTKLMTRLTYTLDAMSGSFKVGSDGSAELKEDDGIDYAATTVQLPGGERV AFLFTIKQFDGKGTLDNIKGDFLVPSYRGSSFLDPKGRGGSTGYDNAVALPARADAEELLKENVKITKALKG SAVFSVAKVDPVTGEIAGVFESIQPSDTDLGAKPPKDIKVTGLWYAQLK*

References

Ferreira, K. N., Iverson, T. M., Maghlaoui, K., Barber, J., & Iwata, S. (2004). Architecture of the Photosynthetic Oxygen-Evolving Center. Science, 303(5665), 1831-1838. doi:10.1126/science.1093087

Mayes, S. R., Cook, K. M., Self, S. J., Zhang, Z., & Barber, J. (1991). Deletion of the gene encoding the Photosystem II 33 kDa protein from Synechocystis sp. PCC 6803 does not inactivate water-splitting but increases vulnerability to photoinhibition. Biochimica et Biophysica Acta (BBA) - Bioenergetics, 1060(1), 1-12. doi:http://dx.doi.org/10.1016/S0005-2728(05)80112-4

Murata, N., & Miyao, M. (1985). Extrinsic membrane proteins in the photosynthetic oxygen-evolving complex. Trends in Biochemical Sciences, 10(3), 122-124. doi:http://dx.doi.org/10.1016/0968-0004(85)90272-5

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