Regulatory

Part:BBa_K1628006

Designed by: Xinhao Song   Group: iGEM15_Nankai   (2015-08-04)
Revision as of 09:07, 18 September 2015 by Songxinhao (Talk | contribs)

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P43

Promoter P43 is a strong promoter in Bacillus subtilis 168. We have validate this part through promoter strength assay using bgaB (encoding β-galatosidase downstream of the multiple cloning sites) as reporter gene. According to our promoter strength assay in (showed in Figure 1), P43 is a weak promoter in B. amyloliquefaciens NK-1.

Promoter4 NK.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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