Composite
Part:BBa_K1804002:Design
Designed by: Tan Yi Han Group: iGEM15_SPSingapore (2015-09-18)
pNirB-Invasin
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 1
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1
Illegal NotI site found at 7 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1
- 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 1
- 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 1
Illegal XbaI site found at 16 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
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Source
The invasin gene from Yersinia is derived from https://parts.igem.org/Part:BBa_K299812 and the pNirB promoter is derived from Jayaraman et al., (1988).
References
The nirB promoter of Escherichia coli: location of nucleotide sequences essential for regulation by oxygen, the FNR protein and nitrite. Molecular Microbioiogy (1988) 2(4), 527-530