Coding
Part:BBa_K1640022
Designed by: Meghan Cook Group: iGEM15_Macquarie_Australia (2015-09-18)
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 537
- 1000COMPATIBLE WITH RFC[1000]
Overview
This biobrick contains a gene from the photosystem II complex of Chlamydomonas reinhardtii - psbP, with RBS included.
This part was designed in conjunction with BBa_K1640012 and BBa_K1640023, together they form operon 5 in our set of photosystem II operons:
Biology & Literature
The psbP gene that comprises this part transcribes a protein that optimizes the availability of Ca2+ and Cl- cofactors in the Oxygen Evolving Complex in PSII to maintain the active Manganese cluster.
Protein information
psbP
mass: 23.17kDa
sequence:
MASGSDVSRRAALAGFAGAAALVSSSPANAAYGDSANVFGKVTNKSGFVPYAGDGFALLLPAKWNPSKENDF
PGVILRYEDNFDAVNNLVVIAQDTDKKAIADFGSQDKFLESVSYLLGKQAYSGETQSEGGFAPNRVSAASLL
DVSTTTDKKGKTYYKYELLVRSADGDEGGRHQLIGATVGSDNKLYIIKIQIGDKRWFKGAKKEAMGAFDSFT
VV*
References
[edit]
Categories
Parameters
None |