Composite
Part:BBa_K1804001:Design
Designed by: Wong Chi Yan Group: iGEM15_SPSingapore (2015-09-17)
GFP under the control of constitutive promoter lacI
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 734
Design Notes
None
Source
The lacI promoter (plac) was obtained from the pAC-EsaR-EsaI plasmid (Shong & Collins, 2013), which was a gift from Cynthia Collins (Addgene plasmid # 47660).
The Green Fluorescence Protein (GFP) gene was obtained from BioBricks Part BBa_E0040 (https://parts.igem.org/Part:BBa_E0040).
References
Shong, J., & Collins, C. H. (2013). Engineering the esaR promoter for tunable quorum sensing-dependent gene expression. ACS synthetic biology, 2(10), 568-575.