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Part:BBa_K1645999

Designed by: Peivand Sadat Mousavi   Group: iGEM15_Waterloo   (2015-09-17)
Revision as of 03:20, 26 September 2015 by Peivandsm (Talk | contribs)

Dual Color Plasmid (RFP and GFP)

This part is a composite of the full GFP cassette (taken from registry part BBa_I20260) and the full RFP cassette (taken from registry part BBa_J04450). The GFP is under the constitutive J23101 promoter. The RFP is under the lacI (R0010) promoter, which is constitutive in DH5-alpha because lacI is not expressed. RFP expression is inhibited if lacI is present in the cell or if IPTG is not present. This part allowed us to visualise colour swaps caused by different SgRNA targeting the promoter region of the two colours colours.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1708
    Illegal AgeI site found at 1820
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 706


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Parameters
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