Composite

Part:BBa_K1806003

Designed by: İBRAHİM YASİR ORHAN   Group: iGEM15_AUC_TURKEY   (2015-09-17)
Revision as of 23:33, 19 September 2015 by TLOS (Talk | contribs)

T7+ ibPB RNA Thermometer + lacI

Designed to close lacO present systems in higher temperatures by producing lacI.

Cloning

The composite part was ligated with the pSB1C3 cloning vector and then verified with Colony PCR. The part was tagged as G-Block 5 and sample 5-6 had the right base pair range. The part was then cut with the EcoR1 and Pst1 restriction enzymes.

6003-1.png 6003-2.png

6003-3.png 6003-4.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None