![](https://parts.igem.org/images/partbypart/icon_device.png)
Part:BBa_K1587004
Butyrate synthesis pathway regulated by constitutive promoter p(Bla)
Constitutive promoter p(Bla). Crotonyl-coA reductase ccr coding region. 3-hydroxybutyryl-CoA dehydrogenase hbd coding region. 3-hydroxybutyryl-CoA dehydratase crt coding region. Acyl-CoA thioesterase 2 tesB coding region. acetyl-CoA acetyltransferase atoB coding region. Every genes are framed by strong RBS based on Ron Weiss thesis. This operon ends with an artificial terminator according to BBa_B1006. Due to a lack of time we decided to have this construction synthesised instead of using the assembly protocol. Unfortunatly NMR analysis did not yield a significant over production of butyrate in E. coli.
![TLSE SHLAGAGA.png](/wiki/images/c/c0/TLSE_SHLAGAGA.png)
![TLSE ButMetabo.jpg](/wiki/images/7/7d/TLSE_ButMetabo.jpg)
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 36
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1717
Illegal BamHI site found at 3634
Illegal XhoI site found at 1407 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 332
Illegal AgeI site found at 1254
Illegal AgeI site found at 2900 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1752
Illegal BsaI.rc site found at 2169
Illegal BsaI.rc site found at 3237
Illegal SapI site found at 123
Illegal SapI.rc site found at 3249
We used BBa_K1587004 in order to produce butyrate
![TLSE Butprod.jpg](/wiki/images/9/99/TLSE_Butprod.jpg)
Figure 1: NMR analysis of butyrate production in E. coli with BBa_K1587004 and wt
We showed no significant production of butyrate using BBa_K1587004. However we observed a statistically significant change in the production of other fermentation products such as ethanol or acetate.
//chassis/prokaryote/ecoli
chassis | E. coli |