Part:BBa_K950001:Experience

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UNIQ53d1617682327bec-partinfo-00000000-QINU UNIQ53d1617682327bec-partinfo-00000001-QINU

••• HUST-China

Pgal1+rox1+cyc1_terminator Figure 1: The whole circuit Modeling Rate of basic transcription for Panb1: Since promoter Anb1 has basic transcription rate, Rox1, Si-tag and Mcfp-3 would be expressed even in darkness. Hence, to figure out how it takes effect on our model, we run the simulation with different rate of Panb1 basic transcription (0.1*Vmax to 0.2*Vmax with step of 0.005*Vmax). Results: Our DDEs model is sensitive to the amount of basic transcription of Panb1. If the rate of pAnb1 basic transcription increases from 0.100*Vmax to 0.200*Vmax, the amount of Rox1 would become 125 percent. Therefore, with less basic transcription of Panb1, less Rox1 would be expressed and the repressed expression of Si-tag and Mcfp-3 would recover more quickly. We can conclude that our circuit could be improved by reducing the basic transcription of pAnb1. Sequence and Features Assembly Compatibility: 10 COMPATIBLE WITH RFC[10] 12 COMPATIBLE WITH RFC[12] 21 INCOMPATIBLE WITH RFC[21] Illegal BglII site found at 1012 23 COMPATIBLE WITH RFC[23] 25 INCOMPATIBLE WITH RFC[25] Illegal AgeI site found at 84 Illegal AgeI site found at 1224 1000 INCOMPATIBLE WITH RFC[1000] Illegal BsaI site found at 1005 Illegal BsaI.rc site found at 1055

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