Coding
HokD-rev

Part:BBa_K1616009

Designed by: Johanna Chesnel   Group: iGEM15_IONIS_Paris   (2015-09-17)
Revision as of 19:10, 17 September 2015 by Chesne j (Talk | contribs)

HokD reversed - RBS and double T7 terminator

The HokD protein is a toxin when overexpressed kills the cells from the inside by interfering with a vital function in the cell membrane. It has been shown that the hokD gene can be used to design efficient suicide functions to contain bacterial growth (Knudsen and Karlstrom 1991, Knudsen, Saadbye et al. 1995)


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



Design Notes

The presence of illegal site have been checked.


Source

This composition part is composed of reversed sequence of the HokD from BBa_K1616008, and RBS reversed BBa_K1616011 and the double terminator T7 reversed BBa_B0025.

References

Knudsen, S., P. Saadbye, L. H. Hansen, A. Collier, B. L. Jacobsen, J. Schlundt and O. H. Karlstrom (1995). "Development and testing of improved suicide functions for biological containment of bacteria." Appl Environ Microbiol 61(3): 985-991.

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