Coding

Part:BBa_K1796010:Design

Designed by: Nannan Xie   Group: iGEM15_SCU_China   (2015-09-17)
Revision as of 02:41, 19 September 2015 by Nieyi (Talk | contribs) (→‎References)

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nifE promoted from Paenibacillus sp. WLY78


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1369
    Illegal PstI site found at 1015
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1369
    Illegal PstI site found at 1015
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1369
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1369
    Illegal PstI site found at 1015
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1369
    Illegal PstI site found at 1015
    Illegal NgoMIV site found at 360
    Illegal NgoMIV site found at 607
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We abtained the sequences from genomes of Paenibacillus sp. WLY78. First, we promoted it ourselvesThen we sent the sequences to synthesis, but unfortunately, striction enzyme cut site was involved after they promoted it again. But the part can be assembled by gibson assembly,that is what we did.


Source

NifE(BBa_K1796010) is taken from genomic sequence of Paenibacillus sp.WLY78, we apply promotions on the sequences and synthesised it.

References

[1]Wang L, Liu Z, Zhao D, Liu X, et al.(2013) A Minimal Nitrogen Fixation Gene Cluster from Paenibacillus sp. WLY78 Enables Expression of Active Nitrogenase in Escherichia coli, PLoS Genet 9(10):e1003865.