Coding

Part:BBa_K1796010

Designed by: Nannan Xie   Group: iGEM15_SCU_China   (2015-09-17)
Revision as of 21:26, 18 September 2015 by Ruifeng (Talk | contribs)

nifE promoted from Paenibacillus sp. WLY78

Function: scaffold


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1369
    Illegal PstI site found at 1015
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1369
    Illegal PstI site found at 1015
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1369
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1369
    Illegal PstI site found at 1015
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1369
    Illegal PstI site found at 1015
    Illegal NgoMIV site found at 360
    Illegal NgoMIV site found at 607
  • 1000
    COMPATIBLE WITH RFC[1000]


Parameter of Protein

Number of amino acids: 1386

Molecular weight: 113485.5

Theoretical pI: 4.98

Amino acid composition:

Ala (A) 349 25.2%

Arg (R) 0 0.0%

Asn (N) 0 0.0%

Asp (D) 0 0.0%

Cys (C) 338 24.4%

Gln (Q) 0 0.0%

Glu (E) 0 0.0%

Gly (G) 383 27.6%

His (H) 0 0.0%

Ile (I) 0 0.0%

Leu (L) 0 0.0%

Lys (K) 0 0.0%

Met (M) 0 0.0%

Phe (F) 0 0.0%

Pro (P) 0 0.0%

Ser (S) 0 0.0%

Thr (T) 316 22.8%

Trp (W) 0 0.0%

Tyr (Y) 0 0.0%

Val (V) 0 0.0%

Pyl (O) 0 0.0%

Sec (U) 0 0.0%

(B) 0 0.0%

(Z) 0 0.0%

(X) 0 0.0%

Total number of negatively charged residues (Asp + Glu): 0

Total number of positively charged residues (Arg + Lys): 0

Atomic composition:Carbon C 4091

Hydrogen H 6798

Nitrogen N 1386

Oxygen O 1703

Sulfur S 338

Formula: C4091H6798N1386O1703S338Total number of atoms: 14316

Extinction coefficients:This protein does not contain any Trp residues. Experience shows that

this could result in more than 10% error in the computed extinction coefficient.

Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water.

Ext. coefficient 21125

Abs 0.1% (=1 g/l) 0.186, assuming all pairs of Cys residues form cystines

Ext. coefficient 0

Abs 0.1% (=1 g/l) 0.000, assuming all Cys residues are reduced

Estimated half-life:The N-terminal of the sequence considered is C (Cys).

The estimated half-life is: 1.2 hours (mammalian reticulocytes, in vitro).

>20 hours (yeast, in vivo).

>10 hours (Escherichia coli, in vivo).

Instability index:The instability index (II) is computed to be 42.28

This classifies the protein as unstable.

Aliphatic index: 25.18

Grand average of hydropathicity (GRAVY): 0.793

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