Coding

Part:BBa_K1796009

Designed by: Nannan Xie   Group: iGEM15_SCU_China   (2015-09-17)
Revision as of 21:20, 18 September 2015 by Ruifeng (Talk | contribs)

nifK promoted from Paenibacillus sp. WLY78

Function: β subunits of dinitrogease which also called FeMo protein.NifK encode a metallocluster: the P-cluster, a [8Fe-7S] cluster which shuttles electrons to FeMo-co.

We abtained the sequences from genomes of Paenibacillus sp. WLY78. First, we promoted it ourselves（done by Nan Wang and Nannan Xie).Then we sent the sequences to synthesis.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 342
    Illegal AgeI site found at 715
    Illegal AgeI site found at 1182
  • 1000
    COMPATIBLE WITH RFC[1000]


Parameter of Protein

Number of amino acids: 516

Molecular weight: 56965.8

Theoretical pI: 5.55

Amino acid composition: 

Ala (A) 49 9.5%

Arg (R) 28 5.4%

Asn (N) 15 2.9%

Asp (D) 29 5.6%

Cys (C) 9 1.7%

Gln (Q) 19 3.7%

Glu (E) 36 7.0%

Gly (G) 43 8.3%

His (H) 17 3.3%

Ile (I) 24 4.7%

Leu (L) 50 9.7%

Lys (K) 21 4.1%

Met (M) 19 3.7%

Phe (F) 23 4.5%

Pro (P) 26 5.0%

Ser (S) 31 6.0%

Thr (T) 28 5.4%

Trp (W) 2 0.4%

Tyr (Y) 19 3.7%

Val (V) 28 5.4%

Pyl (O) 0 0.0%

Sec (U) 0 0.0%

(B) 0 0.0%

(Z) 0 0.0%

(X) 0 0.0%

Total number of negatively charged residues (Asp + Glu): 65

Total number of positively charged residues (Arg + Lys): 49

Atomic composition:Carbon C 2521

Hydrogen H 3935

Nitrogen N 691

Oxygen O 759

Sulfur S 28

Formula: C2521H3935N691O759S28Total number of atoms: 7934

Extinction coefficients:Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water.

Ext. coefficient 39810

Abs 0.1% (=1 g/l) 0.699, assuming all pairs of Cys residues form cystines

Ext. coefficient 39310

Abs 0.1% (=1 g/l) 0.690, assuming all Cys residues are reduced

Estimated half-life:The N-terminal of the sequence considered is A (Ala).

The estimated half-life is: 4.4 hours (mammalian reticulocytes, in vitro).

>20 hours (yeast, in vivo).

>10 hours (Escherichia coli, in vivo).

Instability index:The instability index (II) is computed to be 44.53

This classifies the protein as unstable.

Aliphatic index: 81.16

Grand average of hydropathicity (GRAVY): -0.216

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