Part:BBa_K1769000:Experience

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Results

<style>

div.item {

   /* To correctly align image, regardless of content height: */
   vertical-align: top;
   display: inline-block;
   /* To horizontally center images and caption */
   text-align: center;
   /* The width of the container also implies margin around the images. */
   width: 24%;

}

.caption {

   /* Make the caption a block so it occupies its own line. */
   display: block;
   font-weight:bold;
   font-size:16px;

} </style>

We test this part in BY-2 tobacoo cell line. First we construct this part in vector PSAT6. It has a promoter CAMV35S that act as a consitiutive promoter in plat cells. To see if the dimeric FYVE protein domain really binds to PI3P receptor on the endosome of plant cell, we added a Phosphoinositide-3-kinase(PI3K) inhibitor, wortmannin, that can inhibit PI3K from turning PI3P precursor into PI3P.

The picture on the left shows that after we introduce dimeric FYVE into BY-2 tobacoo cell line, the FYVE concentrated in endosome and cell membrane. However, one hour after we added 10^-6M wortmannin, dimeric FYVE redistributed in cytoplasm.

Oomycete and potato cell

Effector protein bonding with PI3P and entering

User Reviews

UNIQd0facf7bc3a786c2-partinfo-00000001-QINU UNIQd0facf7bc3a786c2-partinfo-00000002-QINU