Part:BBa_K1632006

fim switch[default ON](Tokyo_Tech/R0010)

The fim switch is the promoter containing repeated DNA sequence which is inverted by the Fim recombinase. Therefore, we can control the expression of the gene downstream of the fim switch by adding the Fim recombinase.
We designed the fim switch which has a J23119 promoter(BBa_K1632000) . Also between the promoter and the inverting site, there are two restriction enzyme sites in each front (SalIand BamHI) and back (BglII and MluI)(Fig. 1. Tokyo Tech fim switch (Tokyo_Tech/J23119) design). So the promoter can easily be interchanged. This fim switch is an exmple of that. Except for insertion of restriction enzyme sites, basically, the design of TokyoTech fim switch is similar with wild type fim switch.

Fig. 1. Tokyo Tech fim switch (Tokyo_Tech/J23119) design (Up:on state Down:off state)

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 539
  Illegal BamHI site found at 333
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]