Part:BBa_K1653027

Geraniol(GOH) production. dev

GOH is generated through GPP hydrolysis by geraniol synthase. A MEP pathway has been shown to synthesize IPP and DMAPP efficiently in E. coli. E. coli engineered with geraniol production device (BBa_K1653027) showed different smell as compared with counterpart control (pSB1C3) and WT. This result derived from Questionnaire survey. And then we tried to detect that the geraniol generated by engineered "E. coli" by GC and GC-MS. However, the GOH were not detected. These result may indicate that E. coli with GOH production device produce smaller amouts of than can be detected by GC and GC-MS.
[[http://2015.igem.org/Team:Nagahama/Medal_Parts#Geraniol_.28GOH.29_production_device.28BBa_K1653027.29 here] is our result page.

fig8：Questionnaire survey of fragrance of geraniol A：WT or recombinant（JM109/empty vector） B：recombinant（JM109/GES） Experimental cooperation persons：20 persons Experiment smelling the smell of A and B Experiment collaborators chose a stronger smell by comparing the A and B.

fig9：The results of the questionnaire survey WT was 20% of the total, and recombinant(JM109/GES) was 80% of the total, suggesting that recombinant(JM109/GES) was stronger smell than WT. p-value is assumed fifty‐fifty that normally can occur, and the difference compared to that assumption. In this result, p-value is less than 0.01. This probability is beyond the range that can occur by chance. This experiment indicate that recombinant(JM109/GES) might being synthesize geraniol.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 2878
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 6665
  Illegal BamHI site found at 5993
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 1462
  Illegal AgeI site found at 2174
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 3867