Intermediate

Part:BBa_K299812:Experience

Designed by: Michał Lower   Group: iGEM10_Warsaw   (2010-10-13)
Revision as of 15:11, 17 September 2015 by Xinyi yeo (Talk | contribs) (User Reviews)

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Please enter how you used this part and how it worked out.

Applications of BBa_K299812

User Reviews

UNIQ37981fc3b0fe00b2-partinfo-00000000-QINU

No review score entered. yunting and xinyi from SPSingapore 2015

Our team had trouble sequencing it initially as the full suffix sequence was absent, and primers that were designed based on the documented nucleotide sequence didn't work. We had to use universal primers to sequence the first 1kb, then design new primers for the next kb. Thus we had 3 staggered rounds of sequencing.

The sequencing results point us to a few major and potentially destructive problem:

A) Segment containing Invasin:

UNIQ37981fc3b0fe00b2-partinfo-00000002-QINU


UNIQ37981fc3b0fe00b2-partinfo-00000003-QINU