Generator
GFP_Si4 ge

Part:BBa_K1602026

Designed by: Jasmin Weber, Michelle Zoeller, Marcus Geissler, Stefan Bohn, Simon Linder, Sebastian Barthel   Group: iGEM15_TU_Darmstadt   (2015-09-16)
Revision as of 01:12, 19 September 2015 by N.Hummel (Talk | contribs)

Inducible generator of HisTag-GFP_Si4-Tag fusion protein

cis-Aconitate decarboxylase - cadA

This part is capable of generating the green fluorescent protein (GFP) under the control of a T7 promotor while codon optimized for usage in E.coli (BBa_B0034. Also the sequence of a Histag is fused to the gene sequence, improving the purification yield. Fusing the Si4-tag(BBa_K1028000) to the 3' end of the sequence, enables the proof of functional silica surface binding of the Si4tag.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 711


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Categories
Parameters
None